Crystallizing membrane proteins
نویسندگان
چکیده
منابع مشابه
Crystallizing Membrane Proteins in Lipidic Mesophases. A Host Lipid Screen.
The default lipid for the bulk of the crystallogenesis studies performed to date using the cubic mesophase method is monoolein. There is no good reason however, why this 18-carbon, cis-monounsaturated monoacylglycerol should be the preferred lipid for all target membrane proteins. The latter come from an array of biomembrane types with varying properties that include hydrophobic thickness, intr...
متن کاملCrystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
A detailed protocol for crystallizing membrane proteins by using lipidic mesophases is described. This method has variously been referred to as the lipidic cubic phase or in meso method. The method has been shown to be quite versatile in that it has been used to solve X-ray crystallographic structures of prokaryotic and eukaryotic proteins, proteins that are monomeric, homo- and hetero-multimer...
متن کاملCrystallizing membrane proteins for structure-function studies using lipidic mesophases.
The lipidic cubic phase method for crystallizing membrane proteins has posted some high-profile successes recently. This is especially true in the area of G-protein-coupled receptors, with six new crystallographic structures emerging in the last 3½ years. Slowly, it is becoming an accepted method with a proven record and convincing generality. However, it is not a method that is used in every m...
متن کاملAn antibody library for stabilizing and crystallizing membrane proteins - selecting binders to the citrate carrier CitS.
Co-crystallization of membrane proteins with antibody fragments may emerge as a general tool to facilitate crystal growth and improve crystal quality. The bound antibody fragment enlarges the hydrophilic part of the mostly hydrophobic membrane protein, thereby increasing the interaction area for possible protein-protein contacts in the crystal. Additionally, it may restrain flexible parts or lo...
متن کاملA robotic system for crystallizing membrane and soluble proteins in lipidic mesophases.
A high-throughput robotic system has been developed for crystallizing membrane proteins using lipidic mesophases. It incorporates commercially available components and is relatively inexpensive. The crystallization robot uses standard automated liquid-handlers and a specially built device for accurately and reproducibly delivering nanolitre volumes of highly viscous protein/lipid mesophases. Un...
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ژورنال
عنوان ژورنال: Nature
سال: 1980
ISSN: 0028-0836,1476-4687
DOI: 10.1038/287490a0